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. 2017 Jun 15;28(12):1676–1687. doi: 10.1091/mbc.E16-12-0863

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© 2017 Bottanelli et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Coatomer-positive tubules contain the retrograde marker KDEL receptor. (a) ARF1^EN-Halo cells were electroporated with a KDEL-R-SNAP–encoding plasmid and labeled with 505-BG and SiR-CA for confocal imaging. KDEL-R-SNAP–containing tubules were also decorated by ARF1^EN-Halo (arrows). (c) We found that 1.3 ± 0.3 ARF1^EN-Halo tubules/min also contained KDEL-R, which corresponds to 22 ± 4% of the total ARF1^EN-Halo tubules observed. (b) β-COP^EN-SNAP cells were electroporated with a KDEL-R-GFP–encoding plasmid and labeled with SiR-CA for confocal imaging. Nearly all observed KDEL-R-GFP tubules were positive for Coatomer (arrows). (d) We counted 1.9 ± 1.1 KDEL-R-GFP/ β-COP^EN-SNAP tubules/min, which corresponds to 94 ± 9% of the total number of tubules observed. Error bars represent SD. Scale bars, 10 μm (a), 5 μm (b).