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. 2017 Jun 15;28(12):1701–1711. doi: 10.1091/mbc.E16-12-0846

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© 2017 Lawrimore et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Chromatin response to DNA damage. (A) Top, schematic of a LacO/LacI-GFP–labeled arm locus 240 kb from CEN2 and 4.7 kb from the I-SceI cut site. I-SceI endonuclease is induced on galactose from its site of integration on chromosome VIII (Lobachev et al., 2004). Bottom, representative images in G1 unbudded cells. Green, 240-kb LacO/LacI-GFP; red, SPB labeled with Spc29-RFP. Scale bar, 1 μm. (B) MSD curves of LacO at 240 kb from CEN2 under phleomycin and Zeocin treatment and at a single DSB adjacent to LacO. (C) R_c values with SEM for WT (n = 25), phleomycin-treated (n = 68), Zeocin (n = 23), galactose (no DSB; n = 36), and I-SceI (n = 48) cells. Phleomycin and galactose sDSB (I-SceI) cells have a significant increase over WT (Student’s t test, p = 0.003 and 0.01, respectively). (D) Scatterplots illustrating variance in LacO/LacI position relative to the SPB.