FIGURE 7:

Damage-induced mobility and telomere position depend on the cytoskeleton. (A) MSD curves of LacO at 240 kb from CEN2 in WT (green), plus nocodazole (orange), I-SceI induced (blue), and I-SceI induced plus nocodazole (yellow) cells. (B) R_c values for WT (n = 25), plus nocodazole (n = 61), I-SceI (n = 48), and I SceI plus nocodazole (n = 20) cells. Error bars are SEM. (C) Scatterplots illustrating variance in LacO/LacI position relative to the SPB. (D) Distribution of LacO at TEL3L relative to the nuclear envelope in G1/S cells. The p values for the χ² test were calculated for each data set to a random distribution (dashed line, 33% for each zone). WT (p = 2 × 10⁻⁶, n = 104), plus phleomycin (p = 0.03, n = 102); plus phleomycin plus nocodazole (p = 7 × 10⁻⁵, n = 86), plus nocodazole (p = 3 × 10⁻¹³, n = 78), dhc1Δ (p = 8.21 × 10⁻⁸, n = 86), dhc1Δ plus phleomycin (p = 1.43 × 10⁻¹⁵, n = 87), kar9 (p = 2.78 × 10⁻⁶, n = 75), and kar9 plus phleomycin (p = 7.85 × 10⁻⁹, n = 65). (E) Bar chart of the mean normalized distance of TEL3L array from the nuclear membrane. Distances were normalized to the radius of the nucleus. Error bars are SEM. Data are the same as in D. Asterisks indicate a significant difference between data sets. Students t test; WT vs. WT plus phleomycin, p = 0.009; WT vs. nocodazole, p = 0.04; WT plus phleomycin vs. WT plus phleomycin plus nocodazole, p = 0.001). ns, significant (Student’s t test, p > 0.05). (F) Schematic of LacO at 240 kb from CEN2.