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. Author manuscript; available in PMC: 2017 Jun 13.
Published in final edited form as: ACS Chem Biol. 2017 Feb 13;12(3):611–621. doi: 10.1021/acschembio.6b01089

Figure 6. Chemoenzymatic glycan detection applications.

Figure 6

(A) Imaging of cell-surface glycans with LacNAc. Lec2 cells stained with chloromethyl fluorescein diacetate (CMFDA, green) and unstained Lec8 cells were mixed at a 1:5 ratio and were cultured for 3 days. The cells were treated with 500 mM GDP-FucAz and 30 mU α1–3 FucT for 15 min, then labeled with 20 mM DIFO-647 for 25 min. Top: Alexa Fluor 647 image merged with fluorescein (green) and Hoechst 33342 stain (blue); middle: Alexa Fluor 647 image merged with the Hoechst 33342 image; bottom: the bright-field image. Scale bars: 20 mm. Reprinted from 40 with permission from Wiley-VHC Verlag & Co © 2011. (B) CHoMP LacNAc labeling method applied to 5 μm, FFPE mouse heart, kidney, lung, skin, spleen, and preputial gland tissues, next to serial sections of the same tissues stained with H&E (Hematoxylin and eosin). Green: LacNAc staining; Blue: DAPI nuclear staining. Reprinted from 47 with permission from Wiley-VHC Verlag & Co © 2014.