FIG. 3.

M. bovis BCG-OVApet and M. smegmatis-OVApet induce a mature dendritic cell phenotype. We infected 10⁶ dendritic cells at a multiplicity of infection of 10 with M. bovis BCG-OVApet (grey hashed line) or M. smegmatis-OVApet (bold line) or left them uninfected (thin line) for 2 h. The remaining extracellular bacteria were killed with antibiotic treatment, and the dendritic cells were cultured for 16 h. Dendritic cells were incubated with phycoerythrin-conjugated antibodies to CD40, CD54, CD80, CD86, H2-K^b and I-A^b and analyzed by flow cytometry. DC2.4 cell surface expression (A) and BMDC cell surface expression (B) are shown.