FIG. 5.
SIINFEKL from heat-killed M. bovis BCG-OVApet and M. smegmatis-OVApet is not presented on MHC class I. We infected 105 DC2.4 (A and B) or BMDC (C and D) in 96-well plates with live (solid squares) or heat-killed (open circles) M. bovis BCG-OVApet (A and C) or M. smegmatis-OVApet (B and D) at a multiplicity of infection of 1, 10, 50, or 100 for 2 h at 37°C. The remaining extracellular bacteria were killed by a 2-h treatment with 200 μg of amikacin per ml at 37°C. Dendritic cells were then washed in PBS and cocultured with 105 B3Z cells for 16 h at 37°C. Supernatants were assayed for IL-2 by solid-phase ELISA.