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. 2005 Feb;73(2):784–794. doi: 10.1128/IAI.73.2.784-794.2005

FIG. 8.

FIG. 8.

Lactacystin-treated and TAP1−/− dendritic cells are able to present SIINFEKL peptide with similar efficiencies. We pulsed 105 C57BL/6 BMDC that had been treated with 10 uM lactacystin (▪) or not (×) with increasing concentrations of SIINFEKL for 2 h and washed and cocultured them with 105 B3Z for 16 h at 37°C. IL-2 secretion from B3Z in supernatants is shown (A). C57BL/6 BMDC (▪) and TAP1−/− BMDC (▴) were pulsed with increasing concentrations of SIINFEKL, washed, and cocultured with B3Z cells for 16 h. IL-2 secretion from B3Z in supernatants is shown (B). C57BL/6 splenic dendritic cells (▪) and TAP1−/− splenic dendritic cells (▴) were pulsed with increasing concentrations of SIINFEKL, washed, and cocultured with B3Z cells for 16 h. IL-2 secretion from B3Z in supernatants is shown (C).