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Identifying Streptomyces species for use in functional metagenomics. Phylogenetically diverse Streptomyces species were tested for conjugation and heterologous expression efficiency using a set of metagenomic clones containing minimal PKS genes. Exconjugants from highly transforming strains were plated on rich media to look for color phenotypes. Broth extracts were examined by HPLC to identify species inducing the most heterologous expression events.
