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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Inflamm Bowel Dis. 2017 Jul;23(7):1120–1132. doi: 10.1097/MIB.0000000000001134

Figure 2. Lcn2 produced by LactisLcn2 is bioactive.

Figure 2

(A) Line graph represents the growth curve for E. coli cultured in the presence of cell lysates (5 μg of protein) from LactisLcn2, LactisCon or vehicle control. Myeloperoxidase (MPO, 100 mU) were pre-incubated with enterobactin (Ent, 10 μM) and cell lysates (5 μg of protein) from LactisLcn2 or LactisCon for 5 min before the assay was initiated. (B) Bar graph represents % MPO activity. (C) Orange halo formation indicates siderophore production by ΔfepA E. coli mutant on CAS plate (cultured overnight, 37ºC) in the presence of LactisLcn2, LactisCon or vehicle control at the indicated CFU concentration/ratio. (D) The cellular iron levels were measured in the cell lysates from LactisLcn2 and LactisCon. All experiments were performed in triplicates and are representative of three independent experiments. Results are expressed as mean ± SEM. *p < 0.05.