Figure 2. Lcn2 produced by Lactis^Lcn2 is bioactive.

(A) Line graph represents the growth curve for E. coli cultured in the presence of cell lysates (5 μg of protein) from Lactis^Lcn2, Lactis^Con or vehicle control. Myeloperoxidase (MPO, 100 mU) were pre-incubated with enterobactin (Ent, 10 μM) and cell lysates (5 μg of protein) from Lactis^Lcn2 or Lactis^Con for 5 min before the assay was initiated. (B) Bar graph represents % MPO activity. (C) Orange halo formation indicates siderophore production by ΔfepA E. coli mutant on CAS plate (cultured overnight, 37ºC) in the presence of Lactis^Lcn2, Lactis^Con or vehicle control at the indicated CFU concentration/ratio. (D) The cellular iron levels were measured in the cell lysates from Lactis^Lcn2 and Lactis^Con. All experiments were performed in triplicates and are representative of three independent experiments. Results are expressed as mean ± SEM. *p < 0.05.