Fig. 1.

Ectopically expressed Ank9 localizes to and disrupts the morphology of the ER. HeLa cells expressing GFP-Ank9 (A) or GFP (B) were fixed and screened at 16 h post transfection with GFP antibody and antibody against one of the ER lumenal markers, calreticulin or PDI, or ER transmembrane proteins, calnexin or derlin-1, prior to examination by confocal microscopy. Representative fluorescence images of cells viewed for GFP, ER marker, and merged images plus DAPI, which stains the nucleus, are presented. White arrows denote representative points of GFP and ER marker signal colocalization. Because the cell cycles of the HeLa cells were not synchronized, the timing by which GFP-Ank9 localizes to and disrupts the ER could not be discerned. Results are representative of three independent experiments with similar results. Scale bars, 20 μm.