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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Cell Microbiol. 2017 Feb 3;19(7):10.1111/cmi.12727. doi: 10.1111/cmi.12727

Fig. 10.

Fig. 10

COPB2 levels are unchanged in cells expressing FLAG-Ank9 or infected with O. tsutsugamushi. Whole cell lysates of HeLa cells that were transfected to express FLAG-Ank9 versus FLAG-BAP (A) or that were uninfected or O. tsutsugamushi infected (C) were analyzed by Western blot with antibodies to detect COPB2, GAPDH or β-actin as a loading control, FLAG to confirm FLAG-tagged protein expression, and/or O. tsutsugamushi outer membrane protein A (OmpA). The mean ± SD COPB2 to GAPDH or β-actin ratios for pairs of lysates from at least three separate experiments were determined using densitometry (B and D). ns, not significant.