Table 2.
Candidate Ank9 binding partners identified by yeast two-hybrid
| Protein | Name and Function | + clones |
|---|---|---|
| SKP1a | S-phase kinase associated protein 1; binds F-box in SCF1 ubiquitin ligase complex | 12/26 |
| ERC1 | ELKS/RAB6-interacting/CAST family member; regulatory unit of IKK complex, vesicle trafficking of endosomes to Golgi | 3/26 |
| COPB2 | Coatomer protein complex (COPI) subunit beta 2; vesicular trafficking, Golgi budding, Golgi-to-ER retrograde trafficking | 2/26 |
| PXR1b | Human peroximal targeting signal import receptor (PXR1) | 2/26 |
| EXOC1 | Exocyst complex component 1 (Sec3); subunit of exocyst complex targeting endosomes to plasma membrane | 1/26 |
| CUL1a | Cullin 1; core component of SCF1 ubiquitin ligase complex | 1/26 |
| FEM1B | Fem 1 homolog b (C. elegans); ankyrin repeat-containing protein, mediates apoptosis | 1/26 |
| PIAS4 | Protein inhibitor of activated STAT, 4 (PIASY); E3-type small ubiquitin-like modifier (SUMO) ligase, SUMO tethering factor, transcription co-regulator | 1/26 |
| SNRNP70 | Small nuclear ribonucleoprotein 70 kDa (U1); recognizes pre-mRNA 5′ splice site, essential for spliceosome assembly | 1/26 |
| SP110 | SP110 nuclear body protein; gene transcription activator | 1/26 |
| ZBTB16 | Zinc finger and BTB binding domain containing 16; transcription factor, involved in cell cycle progression | 1/26 |
a
Protein known to interact with Ank9 (Beyer et al., 2015).
b
(Italics) Prey sequences were from an untranslated region of the respective gene, therefore no protein function is listed.