Table 3.
Oligonucleotides used in this study
| Designationa | Sequence (5′ to 3′) |
|---|---|
| β-actin F | AGAGGGAAATCGTGCGTGAC |
| β actin R | CAATAGTGATGACCTGGCCGT |
| ank9 4F | ATCGGAATTCgGGGAGATTCACCAG |
| ank9 141R | ATCGGTCGACTCAGATGTTCCTATCGGCGATAATG |
| ank9 139F | ACGTGAATTCgATCAATATCAATGCCCTGAATG |
| ank9 1266R | ATCGGTCGACTCAGTCGCAGATG |
| Ot 16S 911F | GTGGAGCATGCGGTTTAATTCGATGATC |
| Ot 16S 1096R | TAAGAATAAGGGTTGCGCTCGTTGC |
| Ot TSA56 313F | GACGACGACAAGATAGTTGGCAAAGGCAAAGTGGATAGTCG |
| Ot TSA56 1260R | GAGGAGAAGCCCGGTTAGCTCAGATCAAATTCGGTTTCTTTTTTAGACACTTC |
a
F and R refer to primers that bind to the sense and antisense strand, respectively. Underlined nucleotides correspond to EcoRI (GAATTC) and SalI (GTCGAC) restriction sites used in cloning; bold residues indicate a stop codon; small letters indicate nucleotides added to maintain frame; italicized nucleotides denote ligase-independent cloning tails.