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. 2017 Jun 13;7:3437. doi: 10.1038/s41598-017-03040-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Gem treatment in non-invasive RF pretreated Capan-1 spheroids. Left to right: Brightfield, DAPI and DRAQ7 channels: (a) Untreated spheroid; (b) Spheroid exposed to RF only; (c) Spheroid exposed to 100 μM Gem only for 24 h; (d) Spheroid pretreated with RF and exposed to 100 μM Gem for 24 h.