Table 2.
List Babesia canis specific candidates from culture supernatant derived and membrane-bound fractions.
| Identification | GO-description1 | SP | TM | GPI | AS | Apicomplexan orthologue2 | Transcriptome evidence (FPKM) |
|---|---|---|---|---|---|---|---|
| Specific B. canis secreted soluble proteins: | |||||||
| BcH000240 | Hypothetical protein, conserved | 1 | 6 | 0 | 0 | no (0.7) | |
| BcH000493 | Hypothetical protein [VESA] | 0 | 0 | 0 | 1 | VESA (B. bovis BBOVII004140) | yes (673.5) |
| BcH001113 | Hypothetical protein, conserved | 0 | 0 | 0 | 0 | yes (544) | |
| BcH002928 | hypothetical protein, conserved | 1 | 0 | 0 | 0 | yes (64.2) | |
| BcH003445 | Secreted antigen 3 | 0 | 0 | 0 | 1 | Secreted antigen 3 (B. gibsoni BAH70325) | yes (26.4) |
| BcH000300 | Merozoite surface protein (Bc 28.2) | 1 | 0 | 0 | 0 | B. canis specific | no (4.5) |
| BcH002050 | SET domain-containing protein | 0 | 0 | 0 | 0 | SET- and MYND domain containing protein, putative (B. bigemina BBBOND0302680) | yes (147.6) |
| BcH002986 | hypothetical protein | 0 | 0 | 0 | 0 | yes (60.1) | |
| BcH003048 | Profilin | 0 | 0 | 0 | 0 | Polypeptide of profiling (B. bigemina BBBOND_0104160) | yes (100.6) |
| BcH002141 | Snare protein | 0 | 1 | 0 | 0 | Snare protein, putative (B. bigemina BBOND0107960) | yes (236.6) |
| BcH000974 | Hypothetical protein, conserved | 1 | 0 | 0 | 0 | VESA (B. bovis BBOV_III006920) | no (4.1) |
| BcH000821 | Spectrin repeat superfamily extracellular matrix binding | 0 | 1 | 0 | 0 | VESA (B. bovis BBOV_II004140) | yes (123.2) |
| BcH000175 | Hypothetical protein, conserved | 0 | 0 | 0 | 0 | yes (57.9) | |
| BcH002958 | Hypothetical protein [membrane protein] | 0 | 0 | 0 | 1 | Membrane protein, putative (B. bigemina BBBOND_0105580) | yes (56.4) |
| Specific B. canis host-RBC membrane presented proteins: | |||||||
| BcH000821 | Spectrin repeat superfamily extracellular matrix binding | 0 | 1 | 0 | 0 | VESA (B. bovis BBOV_II004140) | yes (123.2) |
| BcH000300 | Merozoite surface protein (Bc 28.2) | 1 | 0 | 0 | 0 | B. canis specific | no (4.5) |
| BcH000299 | Merozoite surface protein (Bc 28.1) | 1 | 1 | 1 | 0 | B. canis specific | yes (18.3) |
| BcH002699 | Histone h2a | 0 | 0 | 0 | 0 | 41-2-protein antigen precursor, putative (B. bigemina BBBOND_0103700) | yes (31.9) |
| BcH001932 | Hypothetical protein, conserved | 0 | 0 | 0 | 0 | (yes 12.5) | |
| BcH002268 | Hypothetical protein, conserved | 0 | 0 | 0 | 0 | yes (111.9) | |
| BcH001655 | VESA | 0 | 0 | 0 | 1 | VESA (B. bovis BBOV_IV002850) | yes (884.1) |
| BcH001448 | Hypothetical protein, conserved | 0 | 0 | 0 | 0 | no (0.9) | |
| BcH003376 | Secreted antigen 1 | 0 | 1 | 1 | 1 | Secreted antigen 1(B. gibsoni ALE14558) | yes (142.5) |
| BcH000391 | Hypothetical protein, conserved | 1 | 0 | 0 | 0 | yes (331.2) | |
| BcH000895 | Hypothetical protein, conserved | 0 | 0 | 0 | 0 | yes (26.4) | |
GO: gene ontology; SP: signal peptide; TM: transmembrane domain; GPI: AS: alternative secretion: GPI: glycophosphatidylinositol: VESA: variant erythrocyte surface antigen.
1Automated annotation by Blast2GO; [if no Blast2GO annotation could be assigned, best pBlast hit was provided in brackets].
2Other than hypothetical.