Skip to main content
. 2017 Jun 13;7:3420. doi: 10.1038/s41598-017-03744-3

Figure 2.

Figure 2

hrSerpinB3 stimulates oriented migration, but not proliferation, in human LX2 cells and human HSC/MFs. (a,b) Confluent and 24 hr starved LX2 cells (a) or HSC/MFs (b) were left untreated or exposed to either hrSerpinB3 (100 ng/ml) or PDGF-BB (10 ng/ml, positive control) for further 48 hrs and then evaluated for proliferation using the crystal violet assay (see Methods). (c,d) Analysis of oriented migration in the modified Boyden’s chamber assay of LX2 cells (c) or HSC/MFs (d) exposed or not to either hrSerpinB3 (100 ng/ml) or PDGF-BB (10 ng/ml, positive control). Data in bar graphs are expressed as means ± SEM of three independent experiments (**p < 0.01 vs control values). (e) Western blot analysis of activation of ERK1/2, JNK1/2 and Akt signaling pathways in LX2 cells exposed to hrSerpinB3 (100 ng/ml). Confluent and 24 hr starved LX2 cells were exposed to SerpinB3 for the indicated time points and activation of pathways was evaluated in total extracts by analysis of levels of phosphorylated versus un-phosphorylated levels of ERK1/2, JNK1/2 and c-Akt. The cropped gels shows in this Fig. have been run under the same experimental conditions.