TABLE 2.
S. iniae sequences identified by signature-tagged mutagenesisa
| Classification | Mutant | Homologyb | Proposed functionc | % Identity (% homology)/spand |
|---|---|---|---|---|
| Cellular processes | F2-12e | Putative serine protease of the DegP/HtrA family (S. pyogenes, spyM18_2256) | Protease | 68(80)/224 |
| H6-4e | Putative serine protease of the DegP/HtrA family (S. pyogenes, spyM18_2256) | Protease | 76(90)/176 | |
| C7-5 | PurB (S. pyogenes, Spy0036, S. agalactiae, SAG0047) | Adenylosuccinate lyase | 94(98)/238 | |
| E7-1 | Putative dehydrogenase (S. pyogenes, spyM18_0608) | Nucleotide sugar dehydrogenase | 93(100)/48 | |
| G10-11 | Putative hydrolase (S. pyogenes, SpyM3_1547, S. agalactiae, SAG1678) | Haloacid, dehalogenase-like family | 73(85)/146 | |
| Cell envelope | F12-1 | EPS11J (S. thermophilus) | Capsule synthesis | 31(56)/180 |
| E4-5 | EPS11J (S. thermophilus) | Capsule synthesis | 42(62)/105 | |
| G6-8 | EPS11K (S. thermophilus) | Capsule synthesis | 50(69)/208 | |
| G1-6 | Hypothetical protein (B. thethaiotaomicron, BT1178) | Capsule synthesis | 30(57)/59 | |
| C6-1 | Hypothetical protein (B. thethaiotaomicron, BT1178) | Capsule synthesis | 24(48)/161 | |
| E6-9 | EPS5N (S. thermophilus) | Capsule synthesis | 32(52)/283 | |
| D5-5 | EPSU (S. thermophilus) | Capsule synthesis | 32(57)/236 | |
| B9-8 | FmhC (S. aureus) | Cell wall synthesis | 23(40)/256 | |
| Regulators | F5-11e | Putative transcription regulator of the TetR/AcrR family (S. mutans, SMU.1349) | Transcription regulator | 29(49)/171 |
| B9-11e | Putative transcription regulator of the TetR/AcrR family (S. mutans, SMU.1349) | Transcription regulator | 29(49)/171 | |
| Transport or binding | G8-2 | Putative sodium/decarboxylate symporter (S. pyogenes, SPy0324) | Ion transport | 55(68)/218 |
| H5-10 | ABC transporter/lantibiotic transport (Clostridium tetani, CTC00625) | ABC transporter | 64(82)/191 | |
| E12-6 | PTSf system, IIC component (S. agalactiae, SAG1899) | Phosphotransferase system | 79(82)/195 | |
| F10-12 | Mg transport, CorA family (Listeria monocytogenes, LMOh7858_0618) | Mg transport | 45(63)/172 | |
| Transposable elements | A6-2 | Transposase (Lactococcus lactis, umb1) | 98(100)/86 | |
| Conserved hypothetical | A6-6e | S. pyogenes, Spy2215 upstream of htrA | Unknown | 73(85)/159 |
| G8-10e | S. pyogenes, Spy2215 upstream of htrA | Unknown | 73(85)/159 | |
| A4-4e | S. agalactiae, GBS0237 similar to integrase | Unknown | 72(87)/33 | |
| B7-4e | S. agalactiae, GBS0237 similar to integrase | Unknown | 63(77)/88 | |
| D3-10 | S. pyogenes, Spy0316 | Unknown | 85(90)/216 | |
| F6-10 | S. pneumoniae, SP1706 | Unknown | 28(48)/226 | |
| G12-10 | S. agalactiae, gbs1976 | Unknown | 50(73)/52 | |
| H10-9 | Streptococcus mutans, SMU.1349 | Unknown | 36(60)/99 |
Thirteen sequences had no significant homology. DNA flanking the site of insertion showed no significant homology to any sequences found in the databases.
Homology scores with a probability of ≤e−7 were considered significant. Homologies were found by using the NCBI BLASTX translating search program. The highest homology returned for each of our input sequences is listed in the table.
Based on functions assigned to homologous proteins returned in database searches.
Percent identity (percent homology) is at the amino acid level over the length of the span (number of amino acids) shown.
The following pairs of mutants were found to have insertions in the same locus: F2-12 and H6-4, F5-11 and B9-11, A6-6 and G8-10, and A4-4 and B7-4. However, all of these insertions occur within different regions of the protein.
PTS, phosphotransferase system.