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. 2017 Jun 13;7:3377. doi: 10.1038/s41598-017-03216-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Score plot of OPLS model obtained from Culm vs Shoot (A); Leaf vs Shoot (C); Culm vs Leaf (E).Permutation analysis of OPLS-DA models from Culm vs Shoot (B); Leaf vs Shoot (D); Culm vs Leaf (F). Two hundred permutations were performed, and the resulting R² and Q² values were plotted. (Green triangle): R²; (blue square): Q². The green line represents the regression line for R² and the blue line for Q².