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. 2017 Jun 13;7:3393. doi: 10.1038/s41598-017-03414-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of IR on pFAK of 2D and 3D cultured MEF cells. Box plots of the number of molecules per 4 × 4 µm ROI of cells cultured in 2D (A) and 3D (B). Cells were irradiated with 6 Gy and were fixed 15 min and 6 h after irradiation. For 2D cells N = 2 and n = 20, for 3D cells N = 2 and n = 10. Statistical analysis was performed with an ordinary one-way ANOVA. ***p ≤ 0.001 and ****p ≤ 0.0001. NS, not significantly different. If not further noted no significance was detected.