Figure 4.

Characterization of the anti-muPA nanobody Nb7. (a) Determination of the apparent substrate affinity $K_M^{\mathit{app}}$ and turnover number $k_{\mathit{cat}}^{\mathit{app}}$ for muPA hydrolysis of the chromogenic substrate pyro-Glu-Gly-Arg-pNa in the absence or presence of Nb7. Error bars, s.d. (n = 3 independent measurements). (b) Fluorescent spectra of p-aminobenzamidine in the absence or presence of full-length muPA, Nb7, a control nanobody or the active site binding peptide mupain-1. The curves are representatives of three independent measurements.