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. 2017 Jun 13;7:3331. doi: 10.1038/s41598-017-03187-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The representative micro-CT images of the femurs in each group (A) (n = 3 for each group). Analysis of the distal femoral trabecular bone parameters by micro-CT. Tb.BMC (B), TMC (C), BV/TV (D), Tb.N. (E), Tb.Th. (F), Tb.sp. (G), Tb.pf. (H) Data were the means ± SD (n = 3 for each group). ^# P < 0.05 and ^## P < 0.01, vs. the SHAM group. *P < 0.05 and **P < 0.01, vs. the DEX group. The static histomorphometric analysis of femur and tibia by hematoxylin & eosin staining (I) (n = 3 for each group). All images of H&E staining were at ×200 magnifications. Bars: 100 μm. TB: trabecular. BMC and BMD of femur, tibia and vertebra (n = 6 for each group). BMC were determined in each of the five groups (J). BMD was calculated on the basis of BMC (K). Evaluation of biomechanical properties on femur by using a three point blending test (L) (n = 3 for each group), It was calculated from load-displacement curves. ^# P < 0.05 and ^## P < 0.01, vs. the SHAM group. *P < 0.05 and **P < 0.01, vs. the DEX group.