Table 4.
Antibacterial activity of Chr-1 and its variants (in μM) in the presence of different salts at their physiological concentrations against E. coli ATCC 25922.
| Peptide | NaCl | KCl | MgCl2 | NH4Cl | ZnCl2 | FeCl3 | 10% FBS |
|---|---|---|---|---|---|---|---|
| Chr-1 | 8.3 | 4.3 | 8.6 | 4.3 | 4.3 | 4.3 | 17.2 |
| G8P-Chr-1 | 8.6 | 4.3 | 4.3 | 2.0 | 2 | 4.1 | 8.6 |
| G13P-Chr-1 | 8.6 | 4.3 | 4.3 | 2.0 | 4 | 4.1 | 8.6 |
| G18P-Chr-1 | 4.3 | 4.3 | 8.6 | 4.3 | 6.6 | 4.1 | 17.2 |
| G13,18P-Chr-1 | 20 | 6 | 20 | 5 | 12 | 26 | >33 |
The final concentrations of NaCl, KCl, NH4Cl, MgCl2, ZnCl2, and FeCl3 were 150 mM, 4.5 mM, 6 μM, 1mM, 8 μM and 4 μM respectively.