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. 2017 Jun 13;7:3419. doi: 10.1038/s41598-017-03548-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of different bone imaging modalities. (a) Wide field comparison of THG (top left), 3PEF (top middle), SHG (top right), fluorescence confocal (CLSM, bottom left), brightfield transmission (BF, bottom middle) and X-ray nanoCT (bottom right) images acquired on a transverse section of a bovine femur. Some cracks are indicated by green arrowheads in (a). Additional cracks are visible on the confocal and brightfield images that were recorded after the nonlinear images. Scale bar, 50 µm. (b) Zoom in the black rectangle in (a). Collagen fibrils along some of the canaliculi are shown by white arrows on the SHG image. Scale bar, 10 µm. See also Supplementary Movie 3 and 4.