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. 2017 Jun 13;7:3332. doi: 10.1038/s41598-017-03211-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Deletion of TRADD sensitizes to DNA damage agents-induced cell death. (a) TRADD^+/+ (WT) and TRADD^−/− (KO) MEFs were treated with H₂O₂ for 24 hours and then cell viability was analyzed by MTT assay. **P < 0.01; ***P < 0.001 (Student’s t-test). (b) TRADD^+/+ (WT), TRADD^−/− (KO), and TRADD^−/− mTRADD (KO-TRADD) cells were treated with H₂O₂ for 24 hours. Cell viability was analyzed by MTT assay. *P < 0.05; ***P < 0.001 (Student’s t-test). (c) Representative images from treatment with DNA-damaging agents in TRADD wild type and knockout MEFs. TRADD^+/+ (WT) and TRADD^−/− (KO) MEFs were treated with doxorubicin (Doxo) and etoposide (Etopo) for 24 hours and then cell viability was analyzed by MTT assay. **P < 0.01 (Student’s t-test). (d) Representative images rescued with TRADD WT, KO, KO (NES), or KO (Src) in knockout MEFs of TRADD. MTT assay of TRADD WT, KO, KO (NES), and KO (Src) MEFs treated with H₂O₂ and TNF (30 ng/mL) plus CHX (2.5 μg/mL) for 24 hours. *P < 0.05; **P < 0.01; n.s., not significant (Student’s t-test).