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. 2017 Jun 13;7:3418. doi: 10.1038/s41598-017-03511-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytotoxic effect of Grewia tiliaefolia (GT) leaf on A549 cells. (a–c) Cytotoxic effect of different solvent extracts of GT on MCF-7, PC-3 and A549 cells at 48 h by MTT assay. (d) Cytotoxic effect of various concentration of BGT on A549 cells at 24 h and 48 h. (e) LDH leakage in A549 cells during the treatment of BGT at indicated concentrations for 48 h. (f) Morphological changes of A549 cells during BGT treatment after 48 h. The arrow heads indicates that the cells undergoing apoptosis display a series of typical morphological features such as cell shrinkage, membrane blebbing, reduced cell intensity and formation of apoptotic bodies. The data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 vs control group