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. 2017 Jun 13;7:3428. doi: 10.1038/s41598-017-03393-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Composition of HMW-GSs in Xiaoyan 81 and six derivative deletion mutants. Xiaoyan 81 is the wild type progenitor, has all three Glu-1 loci (Glu-A1, -B1 and -D1), and expresses five HMW-GSs (1Ax1, 1Dx2, 1Bx14, 1By15 and 1Dy12). DLGluA1, DLGluB1 and DLGluD1 are single deletion mutants lacking Glu-A1, -B1 and -D1, respectively. DLGluA1B1, DLGluA1D1 and DLGluB1D1 are double deletion mutants lacking Glu-A1/Glu-B1, Glu-A1/Glu-D1 and Glu-B1/Glu-D1, respectively. The HMW-GSs missed in the six mutants are 1Ax1 (DLGluA1), 1Bx14 + 1By15 (DLGluB1), 1Dx2 + 1Dy12 (DLGluD1), 1Ax1 + 1Bx14 + 1By15 (DLGluA1B1), 1Ax1 + 1Dx2 + 1Dy12 (DLGluA1D1), and 1Bx14 + 1By15 + 1Dx2 + 1Dy12 (DLGluB1D1), respectively.