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. 2017 Jun 13;7:3395. doi: 10.1038/s41598-017-03595-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Difference of the ratio r between treated and untreated cells as calculated in the cytosol of A6 epithelial cells. (b) The data reported in (a) can be converted to variation of [Na⁺] or [Cl⁻] in the cytosol using the linear fitting equation reported in the inset of Fig. 3(a). At each z-axis coordinate, the statistically meaningful variations of r and [Na] (or [Cl⁻]) are marked by *(unpaired t-test, 95% confidence, p < 0.05).