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. 2017 Jun 14;9:196. doi: 10.3389/fnagi.2017.00196

Figure 2.

Figure 2

The protective effect of pentazocine on MPP+-induced downregulation of Wnt/β-catenin signaling can be ameliorated by Dickkopf-1 (Dkk1) pretreatment. (A) The immunofluorescence analysis of β-catenin in SN4741 cells. Cells were pretreated with pentazocine (20 μM) for 24 h before 24 h of MPP+ (90 μM) treatment. Moreover, we treated the SN4741 cells with 10 μM naloxone for 15 min or 100 ng/μl Dkk1 for 2 h before adding pentazocine and MPP+. (B) Transfection efficiency of TOPFlash/FOPFlash plasmids in SN4741 cells was assayed by GFP plasmid. (C) SN4741 cells were transfected with TOPFlash/FOPFlash luciferase reporter constructs and were treated as described in (A). Original relative activity means the ratio of luciferase activity in each group compared with the resulting from FOP in MPP+ group. Then the original relative activity/cell viability in each group can be regarded as relative activity. (The means ± SEM; n = 5; *p < 0.05).