TABLE 2.
Strains and plasmids used in this study
| Strains or plasmid | Relevant genotype or phenotype | Source or reference |
|---|---|---|
| V. cholerae | ||
| CVD110 | E7946 El Tor Ogawa Δ(cep-ctxB) hlyA::mer::ctxB Hgr | 15 |
| GOA1264 | Spontaneous Rifr form of CVD110 Rifr | This work |
| GOA1245 | GOA1264 res cassette Rifr Kmr Sucs | This work |
| GOA1246 | GOA1264 res1 cassette Rifr Kmr Sucs | This work |
| GOA6W | GOA1264 lacZ::pGP704 Apr LacZ− | Laboratory strain |
| GOA1745 | GOA1245 irgA::pGOA1196 Rifr Kmr Sucs | This work |
| GOA1746 | GOA1245 irgA::pGOA1197 Rifr Kmr Sucs | This work |
| GOA1747 | GOA1246 irgA::pGOA1196 Rifr Kmr Sucs | This work |
| GOA1748 | GOA1246 irgA::pGOA1197 Rifr Kmr Sucs | This work |
| GOA1737 | GOA1264 ΔVC1500 Rifr | This work |
| GOA1738 | GOA1264 ΔVC2487 Rifr | This work |
| GOA1739 | GOA1264 ΔVC0488 Rifr | This work |
| GOA1740 | GOA1264 ΔVC0622 Rifr | This work |
| GOA1741 | GOA1264 ΔVC0874 Rifr | This work |
| GOA1742 | GOA1264 ΔVC0130 Rifr | This work |
| GOA1743 | GOA1264 ΔVC2705 Rifr | This work |
| GOA1744 | GOA1264 ΔVC2646 Rifr | This work |
| E. coli | ||
| SM10λpir | thi recA thr leu tonA lacY supE RP4-2-Tc::Mu λ::pir Kmr | Laboratory strain |
| DH5αλpir | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR1 supE44 thi-1 gyrA96 relA1::pir | 10 |
| Plasmids | ||
| pRR51 | pBR322::(res-tet-res) | 20a |
| pGP704 | oriR6K mobRP4 Apr | 16 |
| pAC212 | pGP704::neo | Laboratory plasmid |
| pCVD442 | oriR6K mobRP4 sacB Apr Sucs | 5 |
| pSL134 | pPCR-Script::(res1-res1) | Laboratory plasmid |
| pGOA1 | pSL134lacZ::(res1-neo-sacB-res1) | This work |
| pGOA2 | pPCR-Script::(res) | This work |
| pGOA3 | pPCR-Script::(res1) | This work |
| pGOA4 | pPCR-Script::(res-res) | This work |
| pGOA5 | pPCR-Script::(res1-res1) | This work |
| pGOA6 | pGOA4::neo-sacB | This work |
| pGOA7 | pGOA5::neo-sacB | This work |
| pSL111 | pGP704::lacZ | Laboratory plasmid |
| pIVET5 | oriR6K mobRP4 lacZ tnpR Apr | 2 |
| pIVET5n | pIVET5 derivative | This work |
| pRES | pSL111::(res-neo-sacB-res) | This work |
| pRES1 | pSL111::(res1-neo-sacB-res1) | This work |
| pGOA1193 | pIVET5n tnpR | This work |
| pGOA1194 | pIVET5n tnpRmut168 | This work |
| pGOA1195 | pIVET5n tnpRmut135 | This work |
| pGOA1196 | pGOA1193::PirgA | This work |
| pGOA1197 | pGOA1195::PirgA | This work |