FIGURE 3.

Effect on glycolysis and on the Δψ_m. (A) Glycolysis was determined using D-³H(U) glucose as a substrate as described in Methods. The rate of glycolysis for the control incubations was 37.5 ± 2.6 nmoles/h/mg protein (mean ± SEM, n = 35 control incubations). (B) The Δψ_m was assessed in HepG2 cells by TMRM fluorescent staining after drug exposure for 48 h. (C) Isolated mouse liver mitochondria were labeled with [³H]-tetraphenylphosphonium bromide and mitochondrial accumulation of radioactivity was determined. All data are expressed as percentage of control incubations containing 0.1% DMSO. Data represent the mean ± SEM of at least three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01 or ^∗∗∗p < 0.001 versus DMSO control.