Table 6.
The metabolic distinguish of AG in different species.
| Time (min) | Remaining substrate ratio (%) |
|||
|---|---|---|---|---|
| Human | Monkey | Dog | Rat | |
| 0 | 100 ± 0 | 100 ± 0 | 100 ± 0 | 100 ± 0 |
| 5 | 94.8 ± 9.33 | 54.6 ± 6.86 | 90.3 ± 7.88 | 83.5 ± 2.58 |
| 10 | 94.6 ± 9.61 | 24.1 ± 1.46 | 78.4 ± 6.32 | 69.4 ± 2.21 |
| 15 | 87.4 ± 3.93 | 14.4 ± 2.38 | 69.2 ± 7.46 | 57.6 ± 2.76 |
| 20 | 84.3 ± 3.10 | 8.67 ± 1.00 | 66.0 ± 4.69 | 51 ± 2.63 |
| 30 | 83.7 ± 3.34 | 6.78 ± 0.38 | 53.9 ± 5.06 | 39.1 ± 2.76 |
| 60 | 72.8 ± 4.83 | 5.00 ± 1.21 | 36.2 ± 3.68 | 19.3 ± 7.89 |
| 90 | 62 ± 6.36 | 3.69 ± 0.12 | 25.9 ± 3.21 | 15.7 ± 9 |
| ke | 0.00508 | 0.124 | 0.0167 | 0.027 |
| t1/2 (min) | 136 | 5.59 | 41.5 | 25.7 |
Ten microliter AG (100 μM) incubated with different microsome (human, monkey, dog, and rat) at 37°C for 3 min. Hundred microliter samples were collected at 0, 5, 10, 20, 30, 60, and 90 min and detected by LC-MS/MS. (Triplicate assays were performed and results were presented as Mean ± SD).