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. 2017 Jun 14;8:1086. doi: 10.3389/fmicb.2017.01086

FIGURE 1.

FIGURE 1

Generation of NS1 mutant viruses. (A) Schematic of the NS segment from WT H9N2 AIV and the NS mutations. NS1 is translated from the full-length mRNA and is shown on the top of the gene; NEP is translated from spliced mRNA and is shown below the gene. Selected amino acid positions are labeled for NS1 and NEP. NS1-73, NS1-100, NS1-128 express truncated NS1 but intact NEP. (B) NS amplification of the WT and NS mutant viruses. Viral genomes were amplified using RT-PCR and the length of the NS vRNA amplicons was examined using agarose gel electrophoresis. Lane 1, 100 bp marker; Lane 2, WT; Lane 3, NS1-73; Lane 4, NS1-100; Lane 5, NS1-128. (C) Western blot analyses. Whole cell lysates obtained from MDCK cells infected with the mutant and wild-type viruses at an MOI of 1 for 3 h were subjected to SDS-PAGE and western blot analysis. Protein bands were visualized using enhanced chemiluminescence.