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. 2017 Jun 14;8:1086. doi: 10.3389/fmicb.2017.01086

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Copyright © 2017 Chen, Zhu, Yang, Yang, Shi, Qin, Peng and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hemagglutination inhibition titers. Ten chickens were i.n. vaccinated with 1 × 106 log10EID₅₀/0.2 ml of rTX-NS1-128 and were bled at 1-week intervals after vaccination. HI titers in sera were determined with wild-type H9N2 TX and 0.5% chicken red blood cells. The tissues of tracheas and lungs were collected and homogenized before log10 EID₅₀/ml were determined. The mean and standard errors are shown.