Table 3.
Transmission and dose-response study of wild-type H9N2 (rTX) and NS1-truncated H9N2 (rTX- NS1-128) viruses in chickens.
| 3 dpi |
5 dpi |
|||||
|---|---|---|---|---|---|---|
| Virus/Dose (EID50) | Transmission method | Oa | Cb | O | C | Seroconversionc |
| rTX – 107 | Inoculation | 5/5d | 5/5 | 5/5 | 5/5 | 5/5 |
| Contact | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | |
| rTX – 106 | Inoculation | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 |
| Contact | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | |
| rTX – 105 | Inoculation | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 |
| Contact | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | |
| rTX – 104 | Inoculation | 3/5 | 3/5 | 3/5 | 2/5 | 5/5 |
| Contact | 2/5 | 2/5 | 2/5 | 1/5 | 5/5 | |
| rTX – 103 | Inoculation | 0/5 | 0/5 | 0/5 | 0/5 | 5/5 |
| Contact | 0/5 | 0/5 | 0/5 | 0/5 | 5/5 | |
| rTX-NS1-128 - 107 | Inoculation | 5/5d | 5/5 | 5/5 | 5/5 | 5/5 |
| Contact | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
| rTX-NS1-128 – 106 | Inoculation | 4/5 | 0/5 | 3/5 | 0/5 | 5/5 |
| Contact | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
| rTX-NS1-128 – 105 | Inoculation | 0/5 | 0/5 | 0/5 | 0/5 | 3/5 |
| Contact | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
| rTX-NS1-128 – 104 | Inoculation | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
| Contact | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
| rTX-NS1-128 – 103 | Inoculation | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
| Contact | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
aOropharyngeal swab.
bCloacal swab.
cHI titer of chickens 21 days post-inoculation or post-exposure. HI < 4log2 was considered negative.
d# of positive chickens/total chickens used.