Figure 3.

Pre-HSCs Express Distinct Levels of EPCR and Delta-like-4

(A) Expression of EPCR in index-sorted E11.5 AGM-derived Fgd5-ZsGr⁺CD45^+/lo single cells, identifying precursors with HSC potential (pre-HSC clone), hematopoietic colony formation without HSC potential (non-HSC hemogenic clone), or no hematopoietic colony formation (non-hemogenic clone) based on functional potential following AGM-EC co-culture. Dashed box contains EPCR^high-expressing cells highly enriched for pre-HSC clones.

(B) Expression of EPCR and Delta-like-4 (Dll4) in index-sorted E9.5 (left panel) and E10.5 (right panel) P-Sp/AGM-derived Fgd5-ZsGr⁺CD41^lo single cells (see also Figure S2 for additional index-sorting parameters).

(C) Engraftment of hematopoietic cells generated following co-culture on AGM-ECs from E9.5 P-Sp hemogenic endothelium (HE, VE-Cadherin⁺CD41⁻CD45⁻) and hematopoietic progenitor cells (HPC, VE-Cadherin⁺CD41⁺ and/or CD45⁺) sorted based on expression of Dll4. Mice with multilineage engraftment at >16 weeks post-transplant are indicated by red data points. Mice lacking detectable peripheral blood engraftment in one or more lineages are indicated by data points in black.