Figure 1.

mTOR Was Activated in the Bone during Adolescent Growth and by Feeding

(A–C) Immuno-staining of p-S6 on bone sections of 1-day-old (A), 4-week-old (B), or 8-week-old mice (C). The femur bones were decalcified and embedded in paraffin. Antibodies against p-S6 and secondary antibodies conjugated with horseradish peroxidase were used to detect the signal.

(D–F) Starvation inhibited mTOR activation, whereas feeding activated mTOR on bone sections. P1 pups were starved overnight (D) by separating from the mother and then euthanized 2 hr (E) or 8 hr (F) after feeding. The femur bones were frozen sectioned and stained for p-S6. Scale bars, 200 μm (A–F left panels) and 50 μm (A–F right panels).