Figure 2.
Ablation of Tsc1 in Prx1+ MSCs but Not in Dermo1+ or Osx+ OBs Led to Shortened but Broadened Limbs and Joint Deformation
(A) Micro-CT images of the femur bones and the vertebrae (L3 and L4) of 2.5-month-old Prx1-Cre; Tsc1f/f, Dermo1-Cre; Tsc1f/f, and Osx-Cre; Tsc1f/f mice. Upper panel, coronal plane of femur; middle panel, transverse plane of femur; bottom panel, vertebrae.
(B) Quantitation data showed that the length of Prx1-Cre; Tsc1f/f mouse long bones was decreased compared with control mice. N = 15 mice.
(C) H&E staining of the growth plates of 2.5-month-old Prx1-Cre; Tsc1f/f and control mouse femur bones. Right panel: quantitation data of the diameter of chondrocytes. Scale bars, 200 μm (left panel) and 20 μm (middle panel). N = 3 mice (6 views each).
(D) H&E staining of the sections of the knee joint of Prx1-Cre; Tsc1f/f and control mice. Scale bars, 200 μm (upper panel) and 20 μm (lower panel).
(E) Quantitation data showed that the width of the Prx1-Cre; Tsc1f/f mouse femur bones was increased compared with control mice. N = 6 mice.
(F) Quantitation data showed that the width of growth plates of Prx1-Cre; Tsc1f/f mouse femur bones was increased compared with control mice. N = 6 mice.
Error bars represent the SD. ∗p < 0.05; ∗∗p < 0.01.