Figure 5.

Ablation of Tsc1 in Prx1+ MSCs but Not in Dermo1+ or Osx+ OBs Suppressed Osteoclastogenesis and Bone Resorption

(A) TRAP staining of bone sections revealed that femurs of Prx1-Cre; Tsc1^f/f mice, but not Dermo1-Cre; Tsc1^f/f or Osx-Cre; Tsc1^f/f mice showed a decrease in the number of OCs in vivo. Scale bar, 50 μm. Data are from three independent experiments.

(B–D) Prx1-Cre; Tsc1^f/f mice showed a decrease in bone erosion surface (B) (N = 8 mice), the number of OCs (C) (N = 8 mice), and the levels of urine DPD (D) (N = 4 mice).

(E) Tsc1^−/− MSCs showed altered expression of Rankl, M-Csf, and Opg. N = 4 independent experiments.

Error bars represent the SD. ^∗p < 0.05; ^∗∗p < 0.01.