Figure 6.
Ablation of Tsc1 in LysM+ Monocytes or Ctsk+ OCs Led to a Decrease in Bone Resorption
(A) Micro-CT images of the femur bones and the vertebrae (L3 and L4) of LysM-Cre; Tsc1f/f mice, Ctsk-Cre; Tsc1f/f mice, and their respective control mice. N = 3 mice.
(B) The length of femur bones of LysM-Cre; Tsc1f/f mice (left panel) and Ctsk-Cre;Tsc1f/f mice (right panel) were not altered compared with those of control mice. N = 6 mice.
(C) LysM-Cre; Tsc1f/f mice (upper panel) and Ctsk-Cre; Tsc1f/f mice (lower panel) showed an increase in the trabecular bone volume. Right panel: quantitation data. N = 4 mice.
(D) LysM-Cre; Tsc1f/f mice (left panel) and Ctsk-Cre; Tsc1f/f mice (right panel) showed an increase in the number of trabecular bones. N = 4 mice.
(E) LysM-Cre; Tsc1f/f mice (left panel) showed normal trabecular thickness, whereas Ctsk-Cre; Tsc1f/f mice (right panel) showed an increase in the thickness of trabecular bones. N = 4 mice.
(F) LysM-Cre; Tsc1f/f mice (left panel) showed normal numbers of OCs, whereas Ctsk-Cre;Tsc1f/f mice (right panel) showed an increase in the numbers of OCs. N = 6 mice.
(G) TRAP staining on bone sections revealed that femurs of LysM-Cre; Tsc1f/f mice showed normal number of TRAP-positive cells, whereas Ctsk-Cre; Tsc1f/f mice showed an increase in the number of TRAP-positive cells. Scale bar, 50 μm.
(H) LysM-Cre;Tsc1f/f and Ctsk-Cre;Tsc1f/f mice showed a decrease in the levels of urine DPD levels (upper panel) and serum levels of CTX (upper panel). N = 6 mice.
Error bars represent the SD. ∗p < 0.05; ∗∗p < 0.01.