Figure 1.

Expression of GFRα1, RET, and GDNF during Postnatal Cerebellar Development

(A) Sagittal cerebellar sections from P7 Ret^GFP mice stained with antibodies against GFP, GFRα1, and Pax2. Arrowheads indicate earlier born GFP⁺ MLIs (solid) and Pax2⁺ Golgi cells in the IGL (open). EGL, external granule layer; ML, molecular layer; IGL, internal granule layer; WM, white matter. The scale bar represents 100 μm.

(B) Sagittal cerebellar sections from P7 Gdnf^bgal mice stained with β-galactosidase and calbindin antibodies. PCL, Purkinje cell layer. The scale bars represent 100 and 50 μm in bottom corner insets.

(C) Sagittal cerebellar sections from P6 Gfra1^CreERT2;R26^YFP mice, injected with tamoxifen at birth (P0), stained with YFP (1–8), Pax2 (a–f), and somatostatin (SST) (g and h) antibodies and counterstained with DAPI (a, c, e, and f). Arrowheads indicate migrating MLIs (a–f) or Golgi cells (g and h). The scale bar represents 25 μm.

(D) Sagittal cerebellar sections from P19 Gfra1^CreERT2;R26^YFP mice, injected with tamoxifen at P15, stained for YFP (a–d), Pax2 (a and b), and somatostatin (c and d) antibodies and counterstained with DAPI. The scale bar represents 25 μm.

(E) Sagittal cerebellar sections from P95 Gfra1^CreERT2;R26^YFP mice, injected with tamoxifen at P90, stained for YFP (b), and counterstained with DAPI (a). Open arrowheads denote YFP⁺ cells in the midbrain (MB). The scale bar represents 50 μm.