Figure 3.

Hepatic Insulin Resistance Was Strongly Associated with Elevated DAG Content in the Cytosolic Fraction and PKCε Translocation

(A) Hepatic ceramide content in obese subjects with normal hepatic insulin sensitivity or hepatic insulin resistance.

(B) Total hepatic DAG content.

(C) Hepatic DAG content in cytosolic fraction.

(D) Hepatic DAG content in membrane fraction.

(E) Individual DAG species in hepatic cytosol. Note the segmented y axis.

(F) Representative bands show PKCε translocation from cytosol to membrane.

(G) Translocation of PKCε from cytosol (c) to membrane (m) as an index of activation.

Data are mean ± SEM (n = 18–29). ^∗p < 0.05. ^∗∗p < 0.01. ^∗∗∗p = 0.001.