Table 1.
The increase in protease level in microvascular endothelial cells (HMEC-1) during EndMT.
| Protease name | Cell lysates | Cell medium |
|---|---|---|
| Presenilin | xxxx | ---- |
| Neprilysin (CD10) | xxx | ---- |
| Cathepsin C | xxx | x |
| Cathepsin S | xxx | x |
| Cathepsin V | xx | ---- |
| Cathepsin X/Z/P | xx | ---- |
| uPA | xx | x |
| DPPIV (CD26) | ---- | xx |
Proteases are the main enzymes implicated in ECM organization and remodeling. To examine the role of the proteases, other than MMPs, during EndMT in microvascular endothelial cells, we compered the protease protein profile in HMEC-1 versus HMEC-1 treated with TGF-β for 24 h using the proteome profiler antibody array (R&D System, ARY025). In our experiments, we analysed both cell lysates and cell medium to establish protease expression and secretion level, respectively. x indicates upregulation intensity. “----” indicates no changes in intensity between HMEC-1 and HMEC-1 treated with TGF-β.