Figure 1.

MCL inhibits Mtb-induced proinflammatory cytokine secretion. Raw264.7 cells (1 × 10⁶/well) were seeded in 6-well plates overnight and infected with H37Ra at a MOI of 10. Four hours after the infection, cells were washed with sterile PBS and then were treated with different concentrations of MCL (5, 10, and 20 μM) for 12 hr. The concentrations of IL-1β (a) or TNF-α (c) in the supernatants were measured by ELISA, and mRNA expression of IL-1β (b) was detected by qRT-PCR. The concentration of NO (d) in the supernatants was measured with Nitric Oxide Assay Kit. Data are shown as the means ± SD (n≧3). ^∗p < 0.05.