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. Author manuscript; available in PMC: 2018 Feb 5.
Published in final edited form as: Mol Cell Endocrinol. 2016 Oct 14;441:124–133. doi: 10.1016/j.mce.2016.10.014

Fig. 1.

Fig. 1

Histology of Case 1 adrenal. A: Hematoxylin and eosin staining. *: small nodules. **: a large nodule. B: Double-immunostaining for CYP11B2 (blue; in-house polyclonal antibody and 5-bromo-4-chloro-3-indolyl-phosphate with nitro blue tetrazolium) and CYP11B1 (brown; in-house polyclonal antibody and 3,3′-diaminobenzidine) as previously reported (Nishimoto et al., 2010). Numbers without parenthesis indicate areas from which DNAs/RNAs were prepared (DNA/RNA numbers in Table 2 and Supplemental Table 1). Numbers in parenthesis indicate Control# or pAATL# for the corresponding DNA/RNA numbers. C and D: H&E and IHC, respectively: Enlarged images of a representative pAATL with subcapsular APCC-like and inner microAPA-like lesions (pAATL#3) (Table 2), which used for preparation of DNA/RNA 80 and 81, respectively. E: Enlarged image of Sirius Red staining on a serial section used for microdissection. The image area corresponds to the boxed area in Fig. 1D. Scale bars in panels A–B, C–D, and E indicate 1 mm, 100 μm, and 200 μm, respectively.