Figure EV3. Synaptobrevin binds efficiently to the syntaxin1:SNAP25:Munc18‐1 complex, but not to monomeric syntaxin1, SNAP25a or Munc18‐1.

• A, B
  (A) Quantification of synaptobrevin binding to the syntaxin1:SNAP25 complex and the syntaxin1:SNAP25:Munc18‐1 complex as measured by fluorescence anisotropy, and (B) quantification of synaptobrevin binding to the ΔN complex and the syntaxin1: SNAP25:Munc18‐1 complex. Error bars in both (A) and (B) indicate the range of values (n = 3).
• C
  Synaptobrevin does not bind to the monomeric constituents of the syntaxin1:SNAP25:Munc18‐1 complex. No increase in anisotropy was observed upon the addition of unlabeled syntaxin1, SNAP25, or Munc18‐1 to fluorescently labeled synaptobrevin. Note that a twofold excess of the monomers with respect to synaptobrevin was used to perform this reaction.