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. 2017 May 8;36(12):1788–1802. doi: 10.15252/embj.201696270

Figure 6. Cartoon summarizing the different associations between Munc18‐1 and the SNAREs syntaxin1, SNAP25, and synaptobrevin that could exist at the different stages of SNARE complex assembly.

Figure 6

  1. Binary syntaxin1:Munc18‐1 complex with syntaxin in a “closed” conformation.
  2. Ternary complex between Munc18‐1, syntaxin1, and SNAP25. As shown by cross‐linking, the C‐terminal region of the SNAP25 Qb‐SNARE motif is associated with the cleft in Munc18‐1 together with the Habc domain. The syntaxin1:SNAP25:Munc18‐1 complex serves as an efficient acceptor complex for rapid synaptobrevin binding.
  3. Binding of synaptobrevin to the syntaxin1:SNAP25:Munc18‐1 complex leads to the formation of a fully zippered four‐helical SNARE complex, with syntaxin1 being in the open conformation and Munc18‐1 still tethered to the N‐terminal domain of syntaxin1.