Figure 2.

Confirmation of NEUROD1 Overexpression in Neocortical and Striatal Astrocytes

(A) Timeline showing experimental design.

(B) Representative images taken from the cortex and striatum showing AAV9-GFP infection from 1 to 7 dpi. Scale bar, 50 μm.

(C) Western blot showing successful overexpression of NEUROD1 after cloning into the AAV9 backbone.

(D) Timeline showing experimental design.

(E) Representative in vitro images of electroporated cells, 6 days post electroporation, with either AAV9-ND1 + pllU2g-GFP or pllU2g-GFP alone. Scale bar, 50 μm.

(F) Quantification of GFP/Tuj1⁺ cells at 6 days post electroporation. ^∗∗∗p = 0.0002. n = 3 independent experiments.

(G) Timeline showing experimental design.

(H) Representative images from the cortex showing significant overlap of infected astrocytes and NEUROD1 in mice injected with AAV9-NEUROD1, but not AAV9-GFP control, at 5 dpi. Scale bar, 50 μm.