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. 2017 May 11;8(6):1506–1515. doi: 10.1016/j.stemcr.2017.04.013

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Overexpression of NEUROD1 Resulted in Minimal Neuronal Differentiation In Vitro

(A) Timeline showing experimental design.

(B) Representative image of cultured astrocytes showing expression of the astrocyte marker GFAP. Scale bar, 50 μm.

(C) Representative images taken in vitro showing astrocytes infected with lenti-CAG-NEUROD1-UbiC-GFP or lenti-CAG-UbiC-GFP at 7 dpi co-labeled with the immature neuronal marker βIII-tubulin (Tuj1). Arrows denote GFP⁺/Tuj1⁺ cells. Scale bar, 50 μm.

(D) Graph showing the percentage of all astrocytes expressing Tuj1 7 days after infection. n = 4 independent experiments/condition. Data are expressed as mean ± SEM.