Figure 1.

The NuRD Subunit MBD3C Co-purifies with WDR5

(A) Silver stain of MBD3-H3F complex.

(B) Western blot (WB) of purified complex from (A) showing interaction of MBD3 with NuRD subunits (left) and with WDR5 or MLL subunit ASH2L (right).

(C) Western blots for MBD3-H3F or WDR5 upon immunoprecipitation (IP) of each. Asterisk indicates immunoglobulin G (IgG).

(D) Silver stain of MBD3 complex expressing individually H3F-tagged MBD3 isoforms.

(E) Western blot of purified complexes from (D) showing interaction with WDR5 and NuRD subunits.

(F) WDR5 or FLAG immunoprecipitation from individually tagged MBD3C-H3F and MBD3CΔN-H3F ESCs. Asterisks indicate IgG.