Figure 3.

Mbd3c Is Dispensable for ESC Differentiation

(A) Western blots from ESCs differentiated over 10 days. Actin serves as a loading control.

(B) Western blots from primary MEFs reprogrammed to iPSCs.

(C) Schematic of the Mbd3 gene showing the sequence and location of the Mbd3c promoter CpG island (red bar). Light-blue boxes indicate exons. CpGs tested for methylation are highlighted in red.

(D) Pyrosequencing of bisulfite-converted DNA from cells collected at the indicated differentiation time points. Error bars represent the SD of three biological replicates.

(E and F) Western blots of differentiating Mbd3ab and Mbd3abc KO cells (E) and two clonal Mbd3c KO lines (F). WT, wild-type.